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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Identification of Tribbles-1 as a Novel Binding Partner of Foxp3 in Regulatory T Cells
doi: 10.1074/jbc.M112.448654
Figure Lengend Snippet: A, sort parameters for isolation of the human CD4+CD25− (up) and CD4+CD25+CD127− (down) T cells also called regulatory T cells. The left panels show the gate eliminating the CD4− T cells and selected specifically the CD4+ T cells (FSC mean Forward Scatter). The right top panel shows the gate for selecting the CD4+CD25− T cells from the CD4+ fraction previously selected. The right bottom panel shows the gate for selecting the CD4+CD25+CD127− T cells from the CD4+ fraction previously selected. B, sort parameters for isolation of the mouse CD4+CD25− and CD4+CD25high T cells (int mean intermediate). The different populations were isolated after a CD4+ T cell enrichment of mouse splenocytes. The panel displays only CD4+ T cells. CD4− T cells were eliminated from this population by the enrichment procedure and then the population was gated for CD4+ T cells. The left gate shows the sorting of the CD4+CD25− T cells. The right gate shows the sorting of the CD4+CD25high T cells.
Article Snippet: CD4+ T cells were isolated using the
Techniques: Isolation
Journal: The Journal of Biological Chemistry
Article Title: Identification of Tribbles-1 as a Novel Binding Partner of Foxp3 in Regulatory T Cells
doi: 10.1074/jbc.M112.448654
Figure Lengend Snippet: A, quantification of the TRIB1 and Foxp3 mRNA in human CD4+CD25− and CD4+CD25+CD127− T cells. Real-time quantitative RT-PCR analysis for quantification of TRIB1 mRNA (left) and Foxp3 mRNA (middle) in CD4+CD25− and CD4+CD25+CD127− human T cells from seven healthy individuals. Statistical analyses were performed using a non parametric Mann-Whitney test. Analysis of correlation between TRIB1 and FOXP3 in CD4+CD25+CD127− T cells and statistical analysis of correlation were performed with a nonparametric Spearman test (right). B, quantification of the TRIB1 and Foxp3 mRNA in mouse CD4+CD25− and CD4+CD25high T cells. Real-time quantitative RT-PCR analysis of TRIB1 (left) and FOXP3 (right) mRNA in CD4+CD25− and CD4+CD25high mouse T cells of six normal C57Bl6 mice. Statistical analyses were performed using a nonparametric Mann-Whitney test.
Article Snippet: CD4+ T cells were isolated using the
Techniques: Quantitative RT-PCR, MANN-WHITNEY
Journal: The Journal of Biological Chemistry
Article Title: Identification of Tribbles-1 as a Novel Binding Partner of Foxp3 in Regulatory T Cells
doi: 10.1074/jbc.M112.448654
Figure Lengend Snippet: Quantification of TRIB1 and Foxp3 mRNA in human CD4+CD25+CD127− T cells freshly isolated or after activation. Real-time quantitative RT-PCR analysis for quantification of TRIB1 mRNA (A) and FOXP3 mRNA (B) in CD4+CD25+CD127− human T cells from healthy individuals after 0 to 24 h of culture in complete medium associated with recombinant IL-2 (rIL-2), anti-CD3, and anti-CD28 antibodies. Analysis of correlation between TRIB1 and Foxp3 expression with statistical analyses performed using a parametric Pearson test.
Article Snippet: CD4+ T cells were isolated using the
Techniques: Isolation, Activation Assay, Quantitative RT-PCR, Recombinant, Expressing